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Job Radboud University Nijmegen Medical Centre (RUN MC) - Postdoc 'Optimizing monocyte derived DC vaccines'

Specifications - (explanation)

Location Nijmegen
Function typesPostdoc positions
Scientific fieldsHealth
Hours 36.0 hours per week
EducationDoctorate
Translations
About employer Radboud University Medical Center (Radboudumc)
Short link www.academictransfer.com/6943

Job description

'Optimizing monocyte derived DC vaccines'

Towards RNA modified DC vaccines RNA transfection is a unique safe form of ‘gene therapy’ not affecting the genome, but to temporarily modify the function of these non-dividing differentiated cells, including DC. Moreover, vaccination with RNA transfected DCs expressing complete tumor antigens in vivo is effective and safe in cancer patients. In this project, we want to expand on this technology broadly, not only to introduce multiple mRNAs encoding tumor antigens but also mRNAs encoding proteins that improve the functional capabilities of monocyte-derived dendritic cells. In addition to mRNA, also short hairpin RNAs (shRNA) can be succesfully electroporated in DCs. This will allow us to interfere with negative feed back loops that limit the immune potentiating capacity of DC. Ultimately, this should yield DCs with enhanced longevity that are optimally equipped for prolonged stimulation of effector T cells while counteracting the expansion and function of immune inhibitory Tregs.

Requirements

Candidates should have obtained a PhD degree in molecular-cell biology/ Immunology or equivalent. In particular experience with cell culture and interest in immunology / cell biology is of relevance. Techniques: Cell culture, Recombinant DNA technology, Immunological techniques, flow cytometry

  • An independent and well-structured working style
  • Well developed social skills

Conditions of employment

  • Temporary 1-3 years
  • 36 hrs per week
  • Salary level  10: maximal € 3860 gross per month
  • 4 postdoc positions and 1 PhD position are available at the department of Tumor Immunology (www.umcn.nl/til) in the additional information the description of all positions.

Organisation

Radboud University Nijmegen Medical Centre (RUN MC)

Nijmegen www.umcn.nl/ UMC St Radboud

Radboud University Nijmegen Medical Centre (RUN MC) is a leading academic centre for medical science, education and health care. Knowledge forms the heart of our organisation, connecting research, education and patient care. Over 8.500 staff and 3.000 students are committed and ambitious to contribute to the future of health care and medical science.

Driven by knowledge, empowered by people.

Department

Tumor Immunology

The Nijmegen Centre for Molecular Life Sciences (NCMLS) in the Netherlands is a leading European research school providing an outstanding research setting for the Radboud University Nijmegen. The NCMLS focuses on basic science and its translation into pioneering treatments for disease. NCMLS innovations are advanced through technology platforms such as genomics, proteomics, and molecular imaging. Research within the department of Tumor Immunology aims at a better understanding of our immune system using molecular-, cell biological- and immunological- techniques and is centred around dendritic cells (DCs) and regulatory T cells (Tregs) which play an important role in regulating the immune response. In addition, the department translates basic research into clinical application. In particular the development of novel dendritic cell based vaccines to treat cancer patients.

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Specifications - (explanation)

Location Nijmegen
Function typesPostdoc positions
Scientific fieldsHealth
Hours 36.0 hours per week
EducationDoctorate
Translations
About employer Radboud University Medical Center (Radboudumc)
Short link www.academictransfer.com/6943

Job description

'Optimizing monocyte derived DC vaccines'

Towards RNA modified DC vaccines RNA transfection is a unique safe form of ‘gene therapy’ not affecting the genome, but to temporarily modify the function of these non-dividing differentiated cells, including DC. Moreover, vaccination with RNA transfected DCs expressing complete tumor antigens in vivo is effective and safe in cancer patients. In this project, we want to expand on this technology broadly, not only to introduce multiple mRNAs encoding tumor antigens but also mRNAs encoding proteins that improve the functional capabilities of monocyte-derived dendritic cells. In addition to mRNA, also short hairpin RNAs (shRNA) can be succesfully electroporated in DCs. This will allow us to interfere with negative feed back loops that limit the immune potentiating capacity of DC. Ultimately, this should yield DCs with enhanced longevity that are optimally equipped for prolonged stimulation of effector T cells while counteracting the expansion and function of immune inhibitory Tregs.

Requirements

Candidates should have obtained a PhD degree in molecular-cell biology/ Immunology or equivalent. In particular experience with cell culture and interest in immunology / cell biology is of relevance. Techniques: Cell culture, Recombinant DNA technology, Immunological techniques, flow cytometry

  • An independent and well-structured working style
  • Well developed social skills

Conditions of employment

  • Temporary 1-3 years
  • 36 hrs per week
  • Salary level  10: maximal € 3860 gross per month
  • 4 postdoc positions and 1 PhD position are available at the department of Tumor Immunology (www.umcn.nl/til) in the additional information the description of all positions.

Organisation

Radboud University Nijmegen Medical Centre (RUN MC)

Nijmegen www.umcn.nl/ UMC St Radboud

Radboud University Nijmegen Medical Centre (RUN MC) is a leading academic centre for medical science, education and health care. Knowledge forms the heart of our organisation, connecting research, education and patient care. Over 8.500 staff and 3.000 students are committed and ambitious to contribute to the future of health care and medical science.

Driven by knowledge, empowered by people.

Department

Tumor Immunology

The Nijmegen Centre for Molecular Life Sciences (NCMLS) in the Netherlands is a leading European research school providing an outstanding research setting for the Radboud University Nijmegen. The NCMLS focuses on basic science and its translation into pioneering treatments for disease. NCMLS innovations are advanced through technology platforms such as genomics, proteomics, and molecular imaging. Research within the department of Tumor Immunology aims at a better understanding of our immune system using molecular-, cell biological- and immunological- techniques and is centred around dendritic cells (DCs) and regulatory T cells (Tregs) which play an important role in regulating the immune response. In addition, the department translates basic research into clinical application. In particular the development of novel dendritic cell based vaccines to treat cancer patients.