Postdoc in Gene Editing and Human iPSC-derived retinal organoids

Postdoc in Gene Editing and Human iPSC-derived retinal organoids

Published Deadline Location
13 Aug 15 Sep Leiden
Do you have a PhD in biomedical sciences and biochemical, molecular cell biological or neurobiological experience? Are you interested in developing novel gene editing therapies for children and adults with retinal diseases? Then we have an interesting postdoc position for you.

Job description

We performed gene augmentation therapy studies and tested these on human iPSC-derived retinal organoids as models for human retinal disease. We plan to develop novel methods that allow gene correction by editing in photoreceptors and retinal pigment epithelium. There are a multifold of genes that if mutated cause non-syndromic blindness. Each of these retinal disease genes may become nonfunctional due to various mutations in one or two of the many exons or regulatory sequences of a disease gene. You will develop novel gene editing methods to rescue the loss of gene function due to mutations. You will also use other cellular and molecular techniques (FACS, qPCR, microscopy, immunofluorescence).

Summary of the required skills

·        You hold a PhD in Biomedical Sciences

·        You are interested in the development of novel gene editing therapy in human iPSC-derived retinal organoids

·        You can culture human pluripotent stem cells

·        You will work with state-of-the-art equipment and in an innovative academic environment

 

 

Specifications

Leiden University Medical Center (LUMC)

Requirements

We are searching for a highly motivated and experimentally talented scientist with a drive to develop novel therapies for the retina. Preferably, you are familiar with molecular biological research and CRISPR-CAS gene editing therapy on mammalian iPSC-derived organoids. Reporting and publishing the results in high impact scientific journals and at international conferences is expected. We are looking for someone who is flexible and can work well together

Conditions of employment

Fixed-term contract: duration project.

You will be employed on the basis of 36 hours per week. Appointment is for minimal 1 year. Your salary is based on your education and experience and is a maximum of  € 4,481  gross per month for a full time position (scale 10 in the Collective Labour Agreement for University Hospitals).

Employer

LUMC

At the Leiden University Medical Center, we continuously work on improving patient care. We invest in groundbreaking, international research and work with the latest equipment. Together with our team of doctors, medical specialists, teachers, academic researchers and supporting staff, we aim for the best quality in health care, education and international research. And we need you to realize our goals!

Department

Department of Cell and Chemical Biology

The research team Retinal Gene Therapy makes use of the laboratories of the Department of Cell and Chemical Biology (CCB). CCB is equipped with state-of-art facilities for molecular and cell biology, microscopy, chemistry, proteomics, genetics and protein chemistry. Furthermore, the LUMC is known for its innovative academic medical research with an multidisciplinary approach. We break new ground by working together.

Additional information

References

Quinn PM et al. 2019. Human iPSC-derived retinas recapitulate the fetal CRB1 CRB2 complex formation and demonstrate that photoreceptors and Müller glia are targets of AAV5. Stem Cell Reports 12:906-919.

Pellissier LP et al. 2015. Gene Therapy into Photoreceptors and Muller Glial Cells Restores Retinal Structure and Function in CRB1 Retinitis Pigmentosa Mouse Models. Hum Mol Genet 24:3104-3118.

Quinn PM et al. 2017. The CRB1 Complex: Following the Trail of Crumbs to a Feasible Gene Therapy Strategy. Front Neurosci 11:175.

Contact: Jan Wijnholds, associate professor / group leader, Department of Ophthalmology

+31(0)71 526 92 69

j.wijnholds@lumc.nl

Specifications

  • Postdoc positions
  • Health
  • max. 36 hours per week
  • max. €4481 per month
  • University Graduate
  • C.19.MP.EM.17

Employer

Leiden University Medical Center (LUMC)

Learn more about this employer

Location

Albinusdreef 2, 2333 ZA, Leiden

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Application procedure

Appointment is initially for one year, possibly to be extended to up to two years. References will be requested as part of the application procedure. You will also be asked to give a presentation and an assessment will be part of the procedure. Irregular working hours are part of the position.

Apply for this job

Apply for this job

Apply for this job via the employer's website.

This application process is managed by the employer (Leiden University Medical Center (LUMC)). Please contact the employer for questions regarding your application.

Application procedure

Application procedure

Appointment is initially for one year, possibly to be extended to up to two years. References will be requested as part of the application procedure. You will also be asked to give a presentation and an assessment will be part of the procedure. Irregular working hours are part of the position.

Make sure to apply no later than 15 Sep 2019 23:59 (Europe/Amsterdam).