PhD Unravelling genetic origins of platelet function disorders using iPSC model systems

PhD Unravelling genetic origins of platelet function disorders using iPSC model systems

Published Deadline Location
14 Jan 15 Feb Amsterdam
Are you a highly motivated and independent PhD candidate with a master’s degree in biology, (bio)molecular/biomedical sciences or equivalent? Are you responsible, team-oriented with good communication and writing skills in English? Please read further.

Job description

This PhD project is one of the 12 work packages within a large Dutch Science Agenda consortium termed SYMPHONY comprising multiple Dutch research and academic centers (www.symphonyconsortium.nl). SYMPHONY aims to significantly improve treatment for patients with bleeding disorders. The innovations will have significant societal impact both for patient care as well as costs for society. The scientific breakthroughs will lead to a better understanding of underlying mechanisms leading to inter-individual variation in bleeding phenotype, treatment and diagnosis necessity according to severity of disease. You will be based within the iPSC facility in the Department of Hematopoiesis as well as within the SYMPHONY consortium. This department harbors several research groups that study hematopoiesis/immunology in health and disease covering most if not all hematopoietic lineages (>60 postdocs, PhD-students, technicians and group leaders).

Sanquin provides a lively, internationally oriented, scientific environment with excellent facilities. Within the iPSC facility, we reprogram somatic cells from control, genome edited and patient material using various ways to introduce the reprogramming factors (e.g. episomal, Sendai, lentiviral). Within this project we will setup iPSC disease model systems to confirm the causative genetic basis of specific platelet disorders as discovered by other WPs within the consortium. We will employ genome editing to introduce or repair the putative mutations to confirm this causality. Subsequently, we aim to unravel the mechanisms underlying these diseases. A range of standard biochemical assays depending on the mutation(s) identified, coupled with more advanced high throughout techniques (e.g. proteomics, (single cell) RNA-sequencing, ATAC-sequencing) will be employed and integrated to understand the mechanisms behind these diseases. Affected pathways will provide novel targets for therapeutic approaches. Data from this project will show if a specific novel mutation is causative, will provide clues or even solve underlying disease mechanism(s) and identifies possible therapeutic targets.

Specifications

Sanquin Blood Supply Foundation (Sanquin)

Requirements

We ask

  • a highly motivated PhD candidate with a master’s degree in biology, (bio)molecular/biomedical sciences or equivalent;
  • independent, responsible and team-oriented with good communication and writing skills in English;
  • experience in the subject of hematopoiesis and/or induced pluripotent stem cell research as well a programming in R will be a competitive advantage!

Conditions of employment

Fixed-term contract: 4 years.

  • challenging translational and fundamental research project in a multi-disciplinary and enthusiastic international team within a large Dutch consortium;
  • temporary position for 4 years;
  • salary and conditions are conform CAO Sanquin;
  • 8,33% end-of-year bonus; i.e. a thirteenth month in case of full-year employment;
  • partial reimbursement of travel costs;
  • pension plan with Pensioenfonds Zorg & Welzijn (PFZW);
  • at fulltime employment (36 hours) 201 hours of leave per annum.

Employer

Sanquin

For more information on 'Research at Sanquin' please watch the video accessible via this link

Additional information

Information can be obtained from Hanneke Kroneman, Corporate Recruiter, 06 - 50 07 33 16. Does this vacancy trigger you, do you recognize yourself in this profile? Please click the 'apply now'  button which will refer you to an application form of Sanquin. Also the application procedure then accessible on our website will offer further details. 

Curious? Click here to read more about Research.

Sanquin does not accept unrequested help from bureaus for this vacancy. CV's (in whatever shape or form) that are send by mediation bureaus to any employee of Sanquin without a written assignment of a corporate recruiter, will be considered property of Sanquin and Sanquin will therefore not owe compensation in any way. 

Specifications

  • PhD
  • Natural sciences; Health
  • max. 36 hours per week
  • University graduate
  • AT 2486

Employer

Sanquin Blood Supply Foundation (Sanquin)

Learn more about this employer

Location

Plesmanlaan 125, 1066 CX, Amsterdam

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Application procedure

Please send Sanquin a motivation letter (explaining why this specific position interesting for you, how would your skillset fit this research), together with your curriculum vitae both in English. Please send your application accompanied by a cover letter (in English) before the 15th of February 2020. A pre-employment screening will be part of the onboarding procedure.

Apply for this job

Apply for this job

This application process is managed by the employer (Sanquin Blood Supply Foundation (Sanquin)). Please contact the employer for questions regarding your application.

Apply for this job via the employer's website

Application procedure

Application procedure

Please send Sanquin a motivation letter (explaining why this specific position interesting for you, how would your skillset fit this research), together with your curriculum vitae both in English. Please send your application accompanied by a cover letter (in English) before the 15th of February 2020. A pre-employment screening will be part of the onboarding procedure.

Make sure to apply no later than 15 Feb 2020 23:59 (Europe/Amsterdam).