The chromosomes of bacteria and archaea are organized and compacted into a nucleoid. This is achieved by the action of nucleoid-associated proteins that fold the genome by bending, wrapping or bridging it. In our group we investigate, using a quantitative approach, the mechanisms employed by the various proteins that are involved in DNA organization and compaction both from a molecular and a cellular perspective (Peeters et al., Nature Rev Microbiol, 2015; van der Valk et al., eLife, 2017; Henneman et al., PLOS Gen, 2018; Qin et al., Nucl Acids Res, 2020; Henneman et al., Nucl Acids Res, 2020; Dame et al., Nature Rev Gen, 2020).

The proposed project is part of the multidisciplinary research programme “Resolving the fundamental building principles of the genome”. This programme brings together in a consortium the expertise and interests of four groups (Remus Dame, UL; Cees Dekker, TU Delft; Wouter de Laat, Hubrecht; Gijs Wuite, VUA). The overall aim is to establish a molecular understanding of the dynamic spatial organization of genomes and its interplay with transcription. To achieve this aim, the consortium partners intensively collaborate, exchanging expertise and people. The goal of this sub-project is to define by using biochemical and biophysical techniques, including live cell microscopy, the role of known and newly identified DNA binding proteins in bacterial and archaeal chromatin organization, and the mechanisms by which these proteins regulate genes.

• A Master’s degree in Biochemistry, biophysics or equivalent;
• Affinity with Quantitative approaches is required;
• Experience with Live cell microscopy and Genetics is a plus;
• Experience with Instrument building and Programming is a plus
• Excellent proficiency in English;
• Good technical experimental skills;
• Ability to work independently and as a team player in a multidisciplinary environment.

The position is available immediately.

We offer a position for initially one year. After a positive evaluation of the progress of the thesis, personal capabilities, and compatibility, the appointment will be extended by a further three years. Salary range from € 2,325 to € 2,972 gross per month (pay scale P in accordance with the Collective Labour Agreement for Dutch Universities).

Leiden University offers an attractive benefits package with additional holiday (8%) and end-of-year bonuses (8.3%), training and career development and sabbatical leave. Our individual choices model gives you some freedom to assemble your own set of terms and conditions. For international spouses we have set up a dual career programme. Candidates from outside the Netherlands may be eligible for a substantial tax break.

All our PhD students are embedded in the Leiden University Graduate School of Science. Our graduate school offers several PhD training courses at three levels: professional courses, skills training and personal effectiveness.

Diversity
Leiden University is strongly committed to diversity within its community and especially welcomes applications from members of underrepresented groups.

Leiden University

The Faculty of Science is a world-class faculty where staff and students work together in a dynamic international environment. Our people are driven by curiosity to expand fundamental knowledge and to look beyond the borders of their own discipline. The research carried out at the Faculty of Science is diverse, ranging from mathematics, artificial intelligence, computer science, astronomy, physics, chemistry and bio-pharmaceutical sciences to biology and environmental sciences. The faculty has grown strongly in recent years and now has more than 2,300 staff and almost 5,000 students. We are located at the heart of Leiden’s Bio Science Park, one of Europe’s biggest science parks, where university and business life come together. For more information, see the website of the Faculty of Science.

The chemistry and life science research in the Leiden Institute of Chemistry (LIC) is organized around two major research areas: ‘Chemical Biology’ and ‘Energy & Sustainability’. The institute’s research themes illustrate the central position of chemistry between biology, medicine and physics. The various research topics carried out within these themes are ideal for executing interdisciplinary research.