Always wanted to know why plants are resistant to almost all and thus a nonhost for most diseases? Then now there is a challenging project in which you can explore a rare susceptible variant of a nonhost species to discover its underlying gene and mechanism of resistance. Do you want to contribute to improving our knowledge of immunity from a crop-wild relative of lettuce? Please read on, and apply!We are looking for an enthusiastic and highly motivated PhD student to work on resistance against downy mildew in the wild lettuce species,
Lactuca saligna.You will be resolving the genetics of a rare susceptible phenotype in a nonhost species. Downy mildew is a notorious disease for the crop lettuce,
Lactuca sativa. The crop-wild relative,
Lactuca saligna, is completely resistant to downy mildew and considered a nonhost species.
L. saligna and
L. sativa have a fascinating evolutionary relationship, as they diverged about 3 Million years ago, but can still be crossed. Previous interspecific mapping attempts (cross
L. saligna x
L. sativa) for complete resistance were hindered partly by reproductive barriers and showed a complex inheritance of the resistance. A different opportunity to explore immunity in the species
L. saligna presents itself in the exceptional existence of a rare susceptible variant. We like to explore this rare variant and answer the following questions: What gene and mechanism causes this susceptibility ? How can we use this information to identify or design a gene variant(s) resulting in resistance in the crop lettuce?
For this you will use: intraspecific mapping populations of
L. saligna (cross of rare susceptible variant x resistant variant), genotype-phenotype analysis, genetic mapping techniques and potentially allele mining and molecular techniques for functional analysis.
In consultation with our team members of the LettuceKnow subtheme resilience-immunity (see below), other related leads from an analysis of genome-wide association mapping panel of ~500 lettuce lines, may also be explored.
About the LettuceKnow research programThe project is embedded in the subtheme resilience-immunity of the
LettuceKnow (see below for more information and the website for additional vacancies). You will join a team of 18 PhD students, post-docs and technicians working in Utrecht, Wageningen and Leiden.
LettuceKnow (a NWO-TTW perspective program) started in 2019 to investigate key resilience and architecture traits of lettuce. Lettuce is an important component of a healthy diet with an extensive global use. The project aims to identify molecular mechanisms and genes for resilience and architecture. The basis for this is the combination of large-scale transcriptomics of a diversity panels of 500 wild and cultivated Lactuca accessions, cutting-edge phenotyping, powerful integrative bioinformatics & machine learning, and state-of-the-art plant biology. The LettuceKnow team brings together leading scientists in the areas of plant biology, genomics and data sciences to enable the optimal use of big data and discover the genes/alleles underlying important traits. It comprises a diverse research community with a strong focus on resolving major challenges for Dutch lettuce breeders to develop resilient and sustainable varieties that are adapted to changing climate conditions.