Intrusive memories in the form of flashbacks, nightmares and intrusive recollections, are amongst the most devastating symptoms of post-traumatic stress disorder (PTSD). Whereas these memories are extremely vivid and highly emotional, but they lack temporal context, being reexperienced as if they were happening in the present. The declarative content also seems to be fragmented and is characterised by impaired factual recall (e.g., on the surrounding context). As such, PTSD patients seem to be characterized by an aberrant trauma memory.

In this project, part of the VIDI grant awarded to Henckens, we aim to investigate the differences between adaptive vs. maladaptive (aberrant) trauma memory by investigating the memory engram in a mouse model for PTSD. This project focusses on the differences in behavioural recollection, whole brain distribution of the engram, as well as its regulation by epigenetic factors.

Experimentally, you will use an established mouse model for PTSD-induction, which has been shown to reliably induce PTSD-like symptomatology - i.e., hypervigilance, insomnia, compulsivity, and impaired attention and risk assessment - in a subset of mice, whereas others do not show any of these behaviours and are resilient. Contrasting these two behavioural phenotypes, you will investigate the neural ensembles representing the trauma memory engram, its reactivation by varying experimental conditions, together with its epigenetic regulation.

Specifically, you will learn to design, perform, and analyse behavioural animal experiments, as well as molecular tools of immunohistochemistry (and fluorescence imaging), iDISCO+, light sheet imaging and analyses of whole brain neuronal activity patterns. You will collaborate with scientists with varying disciplines, participate in international conferences, and prepare manuscripts for publication as well as a thesis at the end of the project.

You have a master's degree and excellent track record in (medical/molecular) biology, molecular life sciences, biomedical sciences, medicine, neuroscience, or in a related field. You also have experience (and high affinity) with coding (R, Matlab or Python). Your attitude is proactive and goal-directed. You posses good organizational skills.

You also have:

• Interpersonal and communication skills to effectively collaborate and communicate in academia.
• Fluency in written and spoken English.
• The capacity to participate in an interdisciplinary, international, and translational research environment.
• FELASA Certificate to conduct animal experiments (preferred).
• Experience in rodent research; behavioural testing, molecular/cellular neuroscientific techniques (preferred).

Candidates who only partially meet this profile are nonetheless strongly encouraged to apply.

Fixed-term contract: 4 years.

The targeted start date for this position is January 2022, but can be flexible. Working at Radboud university medical center means that you are ahead of the curve and working together on the healthcare of the future. And there is more. Our secondary terms of employment are impressive. These are fully tailored to you thanks to our Employment Conditions Selection Model. At Radboud university medical center, you will be given trust, and you will take the responsibility to handle everything together. We provide annual courses, both professional and personal.

• In addition to your monthly salary and an annual vacation allowance of 8%, you will receive an end-of-year bonus of 8.3%.
• If you work irregular hours, you will receive an allowance.
• As a full-time employee (36 hours per week), you are entitled to approximately 168 vacation hours (over 23 days) per year.
• Radboud university medical center pays 70% of the pension premium. You pay the rest of the premium with your gross salary.
• You get a discount on health insurance as well: you can take advantage of two group health insurance plans. UMC Zorgverzekering and CZ collectief.

In addition to our terms of employment, we also offer employees various other attractive facilities, such as childcare and sports facilities. Want to learn more? Take a look at the Cao UMC.

Radboudumc

The project is part of the research conducted by the group of Dr. Marloes Henckens, assistant professor at the department of Cognitive Neuroscience (CNS) of the Radboudumc, focusing on the neural correlates of differential susceptibility to stress-related disorders, with a specific interest in PTSD.

The project is embedded in the unique and excellent infrastructure of the Donders Institute for Brain, Cognition and Behaviour, a world-class research centre devoted to understanding the mechanistic underpinnings of cognition and behaviour in health and disease. It is a highly dynamic institute with a collaborative atmosphere and particular interest into translational research. For further information, please, visit the following websites: Radboud University Nijmegen; Donders Institute for Brain, Cognition and Behaviour; Department of Cognitive Neuroscience.

Research into stress and stress-related disorders is an important focus area at Radboud university medical center. Researchers working on this theme jointly organize regular discussion meetings and lectures to promote integration of research conducted within molecular, cellular, systems, behavioural, and clinical neurosciences across campus.

Research Institutes
At the moment there are more than 1,300 PhD candidates at our medical hospital. This number includes PhD candidates on our pay roll as well as external candidates (those employed somewhere else but researching on our premises).

• Radboud Institute for Health Sciences: ± 700
• Radboud Institute for Molecular Life Sciences: ± 400
• Donders Center for Medical Neurosciences: ± 200

Read what it is like to do a PhD at the Radboud University Medical Center.

Radboudumc
Radboud university medical center is a university medical center for patient care, scientific research, and education in Nijmegen. Radboud university medical center strives to be at the forefront of shaping the healthcare of the future. We do this in a person-centered and innovative way, and in close collaboration with our network. We want to have a significant impact on healthcare. We want to improve with each passing day, continuously working towards better healthcare, research, and education. And gaining a better understanding of how diseases arise and how we can prevent, treat, and cure them, day in and day out. This way, every patient always receives the best healthcare, now and in the future. Because that is why we do what we do.

Read more about our strategy and what working at Radboud university medical center means. Our colleagues would be happy to tell you about it. #weareradboudumc