We are looking for a PhD candidate 'Human iPSC neuronal platform for neurodevelopmental disorders' to perform research on the development of anti-sense oligonucleotide (ASO) therapy for neurodevelopmental disorders at the Human Genetics Department at the Radboudumc in Nijmegen.

Approximately 1% of children are born with severe neurodevelopmental disorders (NDDs) for which there is currently no disease-modifying therapy. However, because NDDs are frequently caused by identifiable pathogenic mutations, a unique therapeutic opportunity has emerged from recent developments in molecular biology, with the potential to revolutionize the treatment of this group of disorders. We developed an in vitro model to investigate neuronal development, by differentiating hiPSCs into excitatory neurons (iNeurons) and forebrain organoids. We will use patient-derived hiPSCs to model several NDDs that are amenable for ASO therapy. The candidate will develop ASO therapies, and test the efficacy at the molecular and functional level. The functional phenotyping will employ measurements using state or the art high-density microelectrode arrays (MEAs) on 2-dimensional neuronal networks as well as on early developmental stages of brain organoids.

The project is part of an interdisciplinary collaboration within a consortium financed by the ZonMW PSIDER program. The candidate will closely interact with research groups of Prof. Elgersma, Prof. Kushner, dr. de Vrij at Erasmus Rotterdam and Prof. Aartsma, Prof. Room in Leiden. In Nijmegen the research will be located at the Human genetics Department (Nadif Kasri group) and embedded in the Donders Institute for Brain, Cognition and Behaviour of the Radboud University Medical Center.

Our research team at the Radboud university medical center is renowned for its genetic, molecular and electrophysiological research in the field of neurological and neurodevelopmental disorders. Over the past years we have developed unique knowledge and resources, such as the human (patient) induced pluripotent stem cell lines, including the required experimental platforms for in-detail assessment of the derived human neuronal in-vitro network models.

Tasks and responsibilities

• The PhD student will be responsible for generating patient and isogenic hIPSC cell lines for several NDDs
• Designs and performs scientific research
• Works in a team with PhD students, scientists and clinicians
• Discusses, plans and performs research in a stimulating environment
• Works in a highly international research group and is willing to travel to other institutes within Europe.

You are highly motivated to work in an interdisciplinary environment at the cutting edge of science. Excellent interpersonal and communication skills are essential, as is a willingness to work flexibly. You also have a strong background in molecular and cellular neuroscience.

Furthermore:

• Academic degree (MSc) in molecular life sciences, biomedical sciences or a related discipline.
• Experience with cell culture of primary neurons and induced pluripotent stem cells.
• Preferentially has basic experience in electrophysiology.

Fixed-term contract: 4 years.

Working at Radboud university medical center means that you are ahead of the curve and working together on the healthcare of the future. And there is more. Our secondary terms of employment are impressive. These are fully tailored to you thanks to our Employment Conditions Selection Model. At Radboud university medical center, you will be given trust, and you will take the responsibility to handle everything together. We provide annual courses, both professional and personal.

• In addition to your monthly salary and an annual vacation allowance of 8%, you will receive an end-of-year bonus of 8.3%.
• If you work irregular hours, you will receive an allowance.
• As a full-time employee (36 hours per week), you are entitled to approximately 168 vacation hours (over 23 days) per year.
• Radboud university medical center pays 70% of the pension premium. You pay the rest of the premium with your gross salary.
• You get a discount on health insurance as well: you can take advantage of two group health insurance plans. UMC Zorgverzekering and CZ collectief.

In addition to our terms of employment, we also offer employees various other attractive facilities, such as childcare and sports facilities. Want to learn more? Take a look at the Cao UMC.

Radboudumc

The research project will be based at the department of Human Genetics. We provide a stimulating work environment within a group of enthusiastic clinical and non-clinical researchers with a strong national and international research network. The departments house a range of top researchers in the areas of Genetics and Neurosciences. The departments both have a strong track record, with recent publications in high-impact journals (Science, Nature, Nature Genetics). The combined infrastructure is state-of-the-art including innovative genetic technologies (Next-Generation Sequencing, etc.), induced pluripotent stem cells (Nijmegen IPS Technology Center) and a wide range of cellular functional assays.

The PhD student will be enrolled in the PhD program of the Donders Institute of Brain, Cognition and Behaviour, and will be a member of the Department of Human Genetics with an excellent, dynamic, challenging and international working environment in which interdisciplinary research is performed with basic, translational and applied aspects. This position is funded by ZonMw. The position is designed and funded as a 4 year full-time PhD studentship position.

Research Institutes
At the moment there are more than 1,300 PhD candidates at our medical hospital. This number includes PhD candidates on our pay roll as well as external candidates (those employed somewhere else but researching on our premises).

• Radboud Institute for Health Sciences: ± 700
• Radboud Institute for Molecular Life Sciences: ± 400
• Donders Center for Medical Neurosciences: ± 200

Read what it is like to do a PhD at the Radboud University Medical Center.

Radboudumc
Radboud university medical center is a university medical center for patient care, scientific research, and education in Nijmegen. Radboud university medical center strives to be at the forefront of shaping the healthcare of the future. We do this in a person-centered and innovative way, and in close collaboration with our network. We want to have a significant impact on healthcare. We want to improve with each passing day, continuously working towards better healthcare, research, and education. And gaining a better understanding of how diseases arise and how we can prevent, treat, and cure them, day in and day out. This way, every patient always receives the best healthcare, now and in the future. Because that is why we do what we do.

Read more about our strategy and what working at Radboud university medical center means. Our colleagues would be happy to tell you about it. #weareradboudumc