A 0.8-1.0 FTE position (32 - 40 hours) is available for a research technician to support two groups at the Department of Bionanoscience at TU Delft: Kristin Grußmayer and Gijsje Koenderink. Start date as soon as possible. 

The Grußmayer lab develops advanced 3D light microscopy methods to perform quantitative studies in live cells to answer fundamental questions in molecular and cell biology e.g., to understand neurodegenerative diseases. We combine label-free and super-resolution fluorescence microscopy. The Koenderink lab uses light microscopy methods to study the cytoskeleton of mammalian cells and its role in cell mechanics and migration. We use confocal fluorescence microscopy coupled with advanced biophysical methods such as atomic force microscopy. The Grußmayer and Koenderink labs work together in a collaborative project aimed at using new super-resolution methods for resolving cytoskeletal interactions in cells. Both teams are highly interdisciplinary with a mix of scientists with backgrounds ranging from biophysics to optics, often with little training in cell culture work.

You will support both groups with 50:50% of your time. In the Grußmayer lab, you will be responsible for keeping all wet lab activities running smoothly and you will be in charge of mammalian cell culture activities. In the Koenderink lab, you will primarily be responsible for establishing protocols for cloning and production of stable GMO’s  and methods for characterization. Both groups seek help in establishing new dye-labelling strategies for (live-cell) imaging, also in the context of their joint project.

This is a laboratory-based position, but the successful candidate will also be expected to provide input in setting up and optimizing new experimental designs. We seek applicants who are well-organized, diligent, creative, flexible, and curious to learn new techniques. In addition, we look for applicants who enjoy collaborating within a diverse and international team, training scientists and students, and working on varying activities.

Main functions

• Managing wet lab activities (introduction and training of new group members, coordinating ordering, inventory and lab safety)
• Conducting and providing support for cell culture and light microscopy experiments
• Providing input into new experimental designs
• Cloning / Producing stable GMO’s (including optimization of transfection/transduction/lentivirus production, CRISP-Cas technology)
• Characterizing GMO’s using methods such as Western blotting, qPCR, FACS, and proteomics

Essential requirements

             Experience with mammalian cell cultures

             Experience with fluorescence microscopy

             Demonstrated teamworking and trouble-shooting skills

             HBO/ B.Sc. degree or similar and ideally already 1-2 years of experience as research technician/lab manager or scientist

             Proficiency in expressing yourself verbally and in writing in English

It is a plus if you fulfill one (or more) of the following

             Knowledge of super-resolution or other advanced light microscopy techniques

             Familiarity with electronic lab notebook environment and record keeping

             Familiarity with stable cell line generation

             Familiarity with neurobiology/neurodegenerative disease     

European citizenship or a Dutch work permit is required to apply (because of Dutch regulations).

Fixed-term contract: 1 jaar.

• 32-40 (0.8-1.0FTE), the position is for one year with the possibility for extension up to 3 years. The salary depends on background and experience.
• A salary according to the Collective Labor Agreement of the Dutch Universities, plus 8% holiday allowance and an end-of-year bonus of 8.3%.
• An excellent pension scheme via the ABP.
• The possibility to compile an individual employment package every year.
• Discount with health insurers.
• Flexible working week.
• Every year, 232 leave hours (at 38 hours). You can also sell or buy additional leave hours via the individual choice budget.
• Plenty of opportunities for education, training and courses.
• Partially paid parental leave
• Attention for working healthy and energetically with the vitality program.

Delft University of Technology

Delft University of Technology is built on strong foundations. As creators of the world-famous Dutch waterworks and pioneers in biotech, TU Delft is a top international university combining science, engineering and design. It delivers world class results in education, research and innovation to address challenges in the areas of energy, climate, mobility, health and digital society. For generations, our engineers have proven to be entrepreneurial problem-solvers, both in business and in a social context.

At TU Delft we embrace diversity as one of our core values and we actively engage to be a university where you feel at home and can flourish. We value different perspectives and qualities. We believe this makes our work more innovative, the TU Delft community more vibrant and the world more just. Together, we imagine, invent and create solutions using technology to have a positive impact on a global scale. That is why we invite you to apply. Your application will receive fair consideration.

Challenge. Change. Impact!

Faculty Applied Sciences

With more than 1,000 employees, including 135 pioneering principal investigators, as well as a population of about 3,400 passionate students, the Faculty of Applied Sciences is an inspiring scientific ecosystem. Focusing on key enabling technologies, such as quantum- and nanotechnology, photonics, biotechnology, synthetic biology and materials for energy storage and conversion, our faculty aims to provide solutions to important problems of the 21st century. To that end, we train students in broad Bachelor's and specialist Master's programmes with a strong research component. Our scientists conduct ground-breaking fundamental and applied research in the fields of Life and Health Science & Technology, Nanoscience, Chemical Engineering, Radiation Science & Technology, and Engineering Physics. We are also training the next generation of high school teachers and science communicators.

Click here to go to the website of the Faculty of Applied Sciences.