The BIOS Lab-on-a-Chip chair ("Miniaturized systems for biomedical and environmental applications") aims at the research and development of Lab-on-a-Chip (LOC) systems. The applied stem cell technology (AST) group aims making patient-specific replicas of the human body, so that every patient can get tailor-made medicine. Both groups are embedded within both the MESA+ institute for Nanotechnology and the TechMed institute. MESA+ is one of the world's largest nanotechnology research institutes; and it's the largest research institute in this field in the Netherlands. TechMed is the University of Twente's research Centre for Biomedical Technology and Technical Medicine.

Research in this project will be part of the "NXTGEN Hightech, Biomedical Production Technologies" growth fund project funded by the Dutch government. This project is a continuation of our recently published work, see the following links for details:

1. https://doi.org/10.1038/s41378-020-00216-z
2. https://doi.org/10.1063/5.0063428
3. https://doi.org/10.1007/s10544-021-00556-1

• *Job specifications

The field:** Organ-on-Chip (OoC) is a game-changing approach in which human cells are cultured in microfluidic chips simulating and predicting the response of healthy and diseased human tissues. OoC has the potential to revolutionize today's biomedical testing procedures that often involve ethically challenged animal testing and, most importantly, lead to variable results. Despite its promise, OoC adoption is hampered by profound technical and functional limitations. In this multidisciplinary project, engineers and biomedical researchers join forces to overcome these limitations by developing a novel OoC platform based on modular microfluidic building blocks (MFBB) and fluidic circuit boards (FCB) [2].

The aim: High-throughput experimentation is essential to this field, as OoC technology is used to e.g. find new stem cell differentiation protocols [3], to screen new pharmaceuticals, or screen toxicological effects. Our approach to this problem is to use pneumatically actuated valves to address many individual cell culture chambers individually by multiplexing [1]. In this project we want to push multiplexing technology by improving our valve technology. This includes possible development of electrostatically driven valves, and by extending the range of materials we use to fabricate our microfluidic chips.

• We are looking for candidates with a Master's Degree in a relevant field, like e.g. biomedical engineering, electrical engineering, mechanical (micro) engineering or physics. Post-doctoral researchers do not need to apply, we are specifically looking for someone interested in obtaining a PhD degree.
• The candidates should be able to work independently yet able to function in this multi-disciplinary project, and have excellent experimental skills.
• Fluent command of English is strictly required.
• Knowledge or experience in microfluidics, organ on chip, and/or cell culturing is considered as a key advantage.
• We are looking for an engineering-type of personality.
• Knowledge of microfabrication (especially focused on polymer processing and/or MEMS), microfluidics, and a basic background in electronics is preferred.
• Most importantly, the BIOS Lab-on-a-Chip and AST groups in general, are very challenging multidisciplinary environments. It is therefore essential to show willingness to look beyond disciplinary boundaries, to learn new techniques and skills required for this project.

• As a PhD candidate at UT, you will be appointed to a full-time position for four years, with a qualifier in the first year, within a very stimulating and exciting scientific environment;
• The University offers a dynamic ecosystem with enthusiastic colleagues;
• Your salary and associated conditions are in accordance with the collective labour agreement for Dutch universities (CAO-NU);
• You will receive a gross monthly salary ranging from € 2.541,- (first year) to € 3.247,- (fourth year);
• There are excellent benefits including a holiday allowance of 8% of the gross annual salary, an end-of-year bonus of 8.3%, and a solid pension scheme;
• A minimum of 232 leave hours in case of full-time employment based on a formal workweek of 38 hours. A full-time employment in practice means 40 hours a week, therefore resulting in 96 extra leave hours on an annual basis.
• Free access to sports facilities on campus.
• A family-friendly institution that offers parental leave (both paid and unpaid);
• You will have a training programme as part of the Twente Graduate School where you and your supervisors will determine a plan for a suitable education and supervision;
• We encourage a high degree of responsibility and independence, while collaborating with close colleagues, researchers and other staff.