Differentiation and maturation of specific cell types from embryonic or adult stem/progenitor cells is orchestrated by transcriptional regulation of genes that involve serval layers of modules. Growth factors, their signaling pathways, and transcription factors (TFs) are important components of these gene regulatory modules. Cells sense the change in their environment and transmit the message to the nucleus where the message is interpreted by a coordinated action of signal transduction effector proteins and TFs. This cascade and its nuclear interpretation is tightly controlled involving several genes that are organized in different gene regulatory networks (biological circuits). Mutations in components thereof result in developmental abnormalities and also cause chronic diseases, including cancer.

The aim of this PhD project is to deconstruct the regulatory network (biological circuits) of signaling pathways and transcription factors during development and disease. This includes: 1) understanding the dynamics of signaling pathways and TFs in steering stem cell pluripotency exit and commitment to differentiation, 2) elucidating the cross-talk between signaling pathways and TFs, at the transcriptional, protein, and protein-DNA interaction level, 3) Elucidating the crosstalk between different signaling pathways (e.g. BMPs/ Wnts), 4) deciphering the hierarchy of signaling pathways and TFs.

To approach these questions, we use a holistic approach applying existing/emerging state-of-the-art computational and molecular biology techniques and also develop new single cell-omics techniques.
Your responsibilities will include co-designing and performing experiments, data analysis, documenting and reporting results in lab- and departmental meetings and at (inter-)national conferences.

We are looking for an exceptionally talented, highly motivated candidate that holds an MSc degree in Biomedical Science and has a strong interest in Molecular/ Cellular Technologies and Computational Biology. Affinity with Bioinformatics is a plus. You are an effective communicator, combining independent thinking with a collaborative attitude.

Being able to present a certificate of good conduct, a valid proof of identity, diploma's and/ or relevant registration such as BIG/ RGS are conditions for the appointment.

You will receive a temporary position for 4 years. The gross monthly salary is € 2.279 ,- in the 1st year and increases to € 2.919,- in the 4th year (scale OIO). The terms of employment are according to the Collective Bargaining Agreement for Dutch University Medical Centers (CAO UMC).

Erasmus MC

Erasmus MC stands for a healthy population and excellence in healthcare. By conducting groundbreaking work, we aim to push boundaries through leading the way in research, education and healthcare. We have access to the latest equipment and techniques in a state-of-the-art environment.

The Systems Biology of Signaling and Transcription Factors group is part of the Department of Cell Biology, which houses innovative technologies and top-level facilities to support its research programs. The main focus of the department is on transcription regulation and genome organization in (neuro-) developmental and blood cell forming systems in order to understand cell fate determination and differentiation. The department is embedded in the dynamic, internationally competitive research community of over 40 biomedical research labs. The main language of the research community is English. During the PhD training, our students follow specialized lab courses organized by the Graduate School at Erasmus MC.