Although the genetic cause of these muscular dystrophies differs, the pathology is similar. The research that you will be a part of as a PhD student aims to slow down disease progression by inhibiting transcripts of genes that code for proteins that play a crucial role in the disease process in model systems. We thereby hope to improve muscle quality and function, regardless of the type of muscular dystrophy. In the study you will select the best compounds based on cell culture experiments and then test them in animal models. Here you will use functional tests, molecular techniques and various histological techniques to determine the effectiveness and safety of the compounds. The research is funded by the Princes Beatrix Spierfonds (the Dutch muscle funding organisation).

In short

• You hold a master’s degree in a biology or biomedical field

• You have an interest in academic research and acquiring a PhD

• You want to conduct research on Duchenne and Limb-girdle muscular dystrophy

You are a highly motivated student with a completed university MSc degree in Biomedical Sciences, Biology or a similar subject, with a specialization in animal experimental research and/or molecular biology. Holding an animal certificate (Art. 9) is essential and you have affinity and experience with working with mouse models. You possess strong laboratory skills in cell culture and molecular biological techniques (such as PCR, genotyping, qPCR), protein analysis (Western blot and ELISA) and fluorescence and light microscopy. Experience with neuromuscular research is an advantage. You are an enthusiastic candidate who can work independently and on large-scale and multi-disciplinary projects. Furthermore, you are precise and organized in conducting experiments. A flexible interpretation of working hours and good communication and writing skills are also important.

Fixed-term contract: four years.

You will be employed on the basis of 36 hours per week. Appointment is for a maximum of four years, to be completed with a doctoral thesis. Your salary will start with € 2,336 gross per month in the first year, amounting to a maximum of € 2,992 gross per month in the fourth year based on a fulltime position (scale Pro in the Collective Labour Agreement for University Hospitals).

References will be requested and you will be asked to give a presentation as part of the application procedure.

LUMC

At the Leiden University Medical Center, we continuously work on improving patient care. We invest in groundbreaking, international research and work with the latest equipment. Together with our team of doctors, medical specialists, teachers, academic researchers and supporting staff, we aim for the best quality in health care, education and international research. And we need you to realize our goals!

http://www.lumc.nl/

DMD genetic therapy research group of the Human Genetics department

The DMD genetic therapy research group is part of the Human Genetics department. The department conducts fundamental biomedical research with the aim of improving the diagnosis, prevention and treatment of diseases. The research group mainly works on genetic therapies to treat muscle diseases such as Duchenne and Limb-girdle muscular dystrophy. The group works closely with the departments of Radiology and Neurology.