Research Technician neuronal cell culture and antibody generation

We offer a position for a research technician to join our complement team to set up co-cultures of neuronal cells, and the UMAB facility for the generation of new monoclonal antibodies.

A technician (HBO) trained in cellular (cell culture, transfection/transduction, flow cytometry) and molecular biological techniques.

You do not need to have years of experience, as long as you can work accurate and are flexible and like to learn new techniques. Affinity with (long) cell cultures is a must.

You are a teamplayer and are eager to learn. You have a high efficiency and reproducibility level in the lab. A professional level of proficiency in English (written and spoken) is recommended. You can think analytically and have problem-solving skills. You will work in an enthusiastic group of 20 people, post-docs, technicians and PhD students.

The maximum salary for this position (36 - 36 hours) is € 4.214,00 gross per month based on full-time employment.

In addition, we offer an annual benefit of 8.3%, holiday allowance, travel expenses and career opportunities. The terms of employment are in accordance with the Cao University Medical Centers (UMC).

The technician will be part of the Antibody Therapy group led by Professor Jeanette Leusen at UMC Utrecht. You will collaborate closely with our team researching the role of complement in neuroinflammatory diseases. Specifically, you will work alongside the senior technician of the iPCS lab, to establish neuronal co-cultures with iPSC-derived motor neurons, sensory neurons, Schwann cells, and astrocytes. Our methods include iPSC cultures, immunohistochemistry and microscopy to evaluate these cell types. All individual iPSC cultures are operational, and all stainings required to characterize these cell types are in place. The complement team will utilize these co-cultures to further investigate complement activation within various neuroinflammatory disease contexts.
Furthermore, you will work closely with the senior technician of the UMCU Monoclonal antibody facility UMAB. UMAB is the facility of the CTI, Center for Translational Immunology of the UMCU, but also the facility for ONCODE researchers. UMAB can generate new monoclonal antibodies on request, ranging for antibodies used for research, diagnostics or therapy. We use a unique cellular immunization strategy of mice, to produce high affinity, very specific antibodies, Screening of serum and hybridoma titers will be done by FACS (flow cytometry), and hybridomas will be cultured, subcloned end sequenced, so antibodies can be produced recombinantly. All these techniques are up and running within the UMAB facility.