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We performed gene augmentation therapy studies and tested these on human iPSC-derived retinal organoids as models for human retinal disease. We plan to develop novel methods that allow gene correction by editing in photoreceptors and retinal pigment epithelium. There are a multifold of genes that if mutated cause non-syndromic blindness. Each of these retinal disease genes may become nonfunctional due to various mutations in one or two of the many exons or regulatory sequences of a disease gene. You will develop novel gene editing methods to rescue the loss of gene function due to mutations. You will also use other cellular and molecular techniques (FACS, qPCR, microscopy, immunofluorescence).
Summary of the required skills
· You hold a PhD in Biomedical Sciences
· You are interested in the development of novel gene editing therapy in human iPSC-derived retinal organoids
· You can culture human pluripotent stem cells
· You will work with state-of-the-art equipment and in an innovative academic environment
We are searching for a highly motivated and experimentally talented scientist with a drive to develop novel therapies for the retina. Preferably, you are familiar with molecular biological research and CRISPR-CAS gene editing therapy on mammalian iPSC-derived organoids. Reporting and publishing the results in high impact scientific journals and at international conferences is expected. We are looking for someone who is flexible and can work well together
Fixed-term contract: duration project.
You will be employed on the basis of 36 hours per week. Appointment is for minimal 1 year. Your salary is based on your education and experience and is a maximum of € 4,481 gross per month for a full time position (scale 10 in the Collective Labour Agreement for University Hospitals).
At the Leiden University Medical Center, we continuously work on improving patient care. We invest in groundbreaking, international research and work with the latest equipment. Together with our team of doctors, medical specialists, teachers, academic researchers and supporting staff, we aim for the best quality in health care, education and international research. And we need you to realize our goals!
The research team Retinal Gene Therapy makes use of the laboratories of the Department of Cell and Chemical Biology (CCB). CCB is equipped with state-of-art facilities for molecular and cell biology, microscopy, chemistry, proteomics, genetics and protein chemistry. Furthermore, the LUMC is known for its innovative academic medical research with an multidisciplinary approach. We break new ground by working together.