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Within the Laboratory of Proteomics we are looking for a candidate to elucidate the interactions between cytokines, leukocytes and the vasculature from an omics based perspective using relevant in vitro models.
Endothelial cells line the inside of our blood vessels and form a barrier that controls immune cell exit from the circulation to the tissues. This is a highly regulated processes and critical for maintaining homeostasis. Endothelial cells need to integrate a wide variety of cytokine signals from their environment and act accordingly. A misbalanced response herein has been suggested to underlie multifactorial diseases, such as sepsis, systemic inflammatory response syndrome (SIRS) and (non)immune-mediated transfusion-related acute lung injury (TRALI). Current knowledge is limited to a select set of cytokines (most notably TNFα). Therefore the goal of this project is to elucidate how the endothelial proteome responds to a wide variety of cytokines, how this affects intracellular signaling cascades and what the outcome is on selective leukocyte trafficking.
This project calls for primary endothelial cell culture, immune cell isolation from blood, proteomics, phospho-proteomics, imaging as well as performing functional assays probing endothelial barrier function and leukocyte trafficking in 3D culture systems. Through this project you will provide an unbiased integrated analysis of the effect of microenvironments on the adaptive endothelial response at multi-omic and functional levels.
We offer unique research opportunities in a dynamic environment. Nowhere else in the world you will find an organization that combines medical, pharmaceutical, diagnostic and scientific research under one roof. In addition to a challenging project in a multi-disciplinary and enthusiastic team we offer:
The Laboratory of Proteomics is embedded in the department of Molecular and Cellular Hemostasis and is a frontrunner in applying state-of-the-art mass spectrometry-based proteomics within the scientific framework of Sanquin Research. We are a vibrant, international group of 35-40 colleagues (consisting of group leaders, postdocs, PhD students and technicians). Our department has academic affiliations with high-ranking universities in the Netherlands, such as Utrecht University and the University of Amsterdam. The department’s research program benefits from excellent research facilities, which include the latest technology for biomolecular mass spectrometry, confocal imaging, high-dimensional flow cytometry and FACS/MACS cell sorting.
Sanquin Blood Supply Foundation (Sanquin)
Plesmanlaan 125, 1066 CX, Amsterdam
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