The project

The Postdoc will be responsible for the design, production and functional testing of intracellularly expressed antibodies, formulated as mRNA transfection vehicles and targeted at oncogenic signaling pathways involved in immune suppression or at spliceosome constituents. Ultimate aim is to enhance the immunogenicity of cancer and so support immunotherapy approaches.

The project is part of a research consortium, funded by the European Union Research and Innovation Actions: HORIZON-HLTH-2021-TOOL-06-02. The CANCERNA consortium brings together molecular biologists and immunologists from six countries in a concerted effort to exploit mRNA-based approaches and mRNA processing modulation in cancer immunotherapy.

About your role

Your main tasks and responsibilities are:

• To screen for, identify, and clone genes encoding targeted antibody fragments;
• To set up and operationalize in-vitro transcription of antibody mRNAs;
• To set up mRNA-based transfection protocols for intracellular antibody expression in tumor cells;
• To carry out flowcytometric, transcriptional, and functional profiling of intracellular antibody-modulated tumor; cells and study their effects on immune effector cells;
• To analyze and write reports and papers on the obtained data.

About you

We are looking for a highly motivated and enthusiastic postdoc:

• With a PhD degree in Biomedical Sciences or Biochemistry and with affinity for Immunology;
• With experience with molecular biology techniques, specifically in-vitro mRNA transcription and transfection;
• You have a flexible, pro-active team spirit but you can also work independently;
• You have excellent communication skills in English, both written and verbal.

Fixed-term contract: 3 years.

Our offer

• The intended start date for the project is ASAP. We offer a fixed term contract for 3 years.
• Besides a good basic salary, you will also receive an 8.3% year-end bonus and 8% holiday allowance. Calculate your net salary here.
• Free and unlimited access to our online learning environment GoodHabitz.
• Pension accrual with ABP, of which we pay 70% of the premium.
• Reimbursement of 75% of your public transport costs. Would you rather travel by bike? Then we have a good bicycle scheme.
• An active personnel association and the Young Amsterdam UMC association, both of which organise fun (sports) activities and events.

• For an overview of all our other terms of employment, see https://werkenbij.amsterdamumc.org/

Bekijk alle arbeidsvoorwaarden van Amsterdam UMC

What to expect

• A job in our beautiful capital city
• Working for the favorite employer in healthcare (intermediary 2021)
• All colleagues have the same goal: contributing to tomorrow’s care
• There is room for development, deepening and broadening of your knowledge
• An active staff association that organizes fun activities and arranges discounts on products and services for you
• Amsterdam UMC innovates continuously, which requires a flexible attitude from everyone
• Two large locations, good for your pedometer!
• You have more than 15.000 colleagues, not all of whom you will get to know personally

Amsterdam UMC

About your workplace

As Potsdoc researcher you will be working at the Amsterdam UMC (location VUmc) in the Immunotherapy Lab of the Cancer Center Amsterdam (CCA) under supervision of Prof Tanja de Gruijl and Prof Hans van der Vliet.

Research within the CCA Immunotherapy Lab is performed in a stimulating environment and at an international highly competitive level. You will be part of a highly motivated team of (pre-) clinical researchers. The focus of research is on the tumor microenvironment and cancer immunotherapy.

Amsterdam UMC has an open culture. This means that we hope that everyone feels welcome in our organization and that we strive to offer equal opportunities to everyone. We therefore cordially invite all interested parties to respond to this vacancy. Check out our approach to diversity and inclusion.

Meet your colleagues. Watch video’s of your future colleagues:

• Amsterdam UMC
• Tulp
• Verpleegkundige visie