As a postdoctoral research fellow you will conduct a research project studying the role of BRCA1 protein complexes in homologous recombination. For this, you will investigate the architecture of the different complexes via cross-linking mass spectrometry (XL-MS) and you will study the temporal and spatial recruitment of these complexes to chromatin upon DNA double-strand break induction via ChIP-sequencing and proteomics approaches.
In short:

• You received your PhD in molecular biology or a related field;

• You have experience in DNA damage repair research;

• You have experience in big data analysis (genomics and/or proteomics);

• You work independently and have excellent communication skills;

• You are motivated to study BRCA1 function in the maintenance of genomic stability.

You are an enthusiastic and ambitious scientist who has obtained a PhD in DNA damage repair or a related field and you have proven scientific excellence with a strong track record. You have experience in standard molecular biology tools (molecular cloning, cell culture, Western blotting, fluorescent microscopy, flow cytometry, etc). You also have experience with CRISPR/Cas9-mediated genome editing and/or protein mass spectrometry and you have affinity with the bio-informatic analysis of these types of big datasets. You have a flexible attitude, great sense of teamwork and possess excellent communication skills.

Fixed-term contract: 1 year.

You will be employed on the basis of 36 hours per week. Your salary is based on your education and experience and is a maximum of  € 4,361  gross per month for a full time position (scale 10 in the Collective Labour Agreement forUniversity Hospitals).

LUMC

At the Leiden University Medical Center, we continuously work on improving patient care. We invest in groundbreaking, international research and work with the latest equipment. Together with our team of doctors, medical specialists, teachers, academic researchers and supporting staff, we aim for the best quality in health care, education and international research. And we need you to realize our goals!

http://www.lumc.nl/

Department of Human Genetics

The Department of Human Genetics is a broadly oriented research department, led by Professor Silvère van der Maarel, focusing on the genetic aspects of a variety of diseases, such as cancer, and the translation into clinical relevance. The department consists of 22 research units, embedded in four overarching research themes. You will work in the recently established research group led by Dr. Sylvie Noordermeer, which is embedded in the research theme ‘Genome instability and cancer’ and has a clear focus on better understanding the fundamental mechanisms of DNA damage repair to improve clinical management of cancer patients. More specifically, the research in the Noordermeer lab governs the study of the protein BRCA1 and its regulation and function in multiple protein complexes involved in homologous recombination and genome stability maintenance. We complement our fundamental research in mammalian cell line models with studies on clinical data of BRCA1-mutated tumours to enable better risk prediction of the wide variety of BRCA1 mutations present in tumours. We have previously uncovered mechanisms involved in therapy resistance of BRCA1-deficient cells to PARPi (Noordermeer et al., Nature, 2018).