Cellular development and differentiation is a tightly controlled process that is orchestrated by the transcriptional regulation of genes. The control of gene transcription entails several layers of regulatory modules. Signaling pathways and their downstream TFs are important components of this gene transcription regulatory module and allow cells to properly respond to environmental cues. This interpretation within the cell's nucleus involves several genes that are organized in gene regulatory networks (GRNs), driving epigenomic and transcriptional changes and thereby cell fate, differentiation and maturation. We are interested in understanding the dynamics of such biochemical cascades and connected GRNs using in embryonic stem cells as a model. The aim of this PhD project is to understand the crosstalk and dynamics of signaling and TFs and their impact on the epigenome. To achieve this, we are using a holistic approach based on perturbation approaches and apply existing/emerging state-of-the-art computational and molecular biology techniques, including the development of novel single cell-omics techniques. Your responsibilities will include co-designing and performing such experiments, analyzing data, and documenting and reporting results in lab- and departmental meetings and at (inter-)national conferences.

We are looking for an exceptionally talented, highly motivated candidate that holds an MSc degree in Biomedical Science or Nanobiology and has a strong interest in biomedical research with stem cells, molecular/cellular technologies and computational biology. Affinity with bioinformatics is therefore a strong plus. In addition, you are an effective communicator, combining independent thinking with a collaborative and team-spirit attitude.

Being able to present a certificate of good conduct, a valid proof of identity, diploma's and/ or relevant registration such as BIG/ RGS are conditions for the appointment.

You will receive a temporary position for 4 years. The gross monthly salary is € 2.357,- in the 1st year and increases to € 3.020,- in the 4th year (scale OIO). The terms of employment are according to the Collective Bargaining Agreement for Dutch University Medical Centers (CAO UMC).

Erasmus MC

Erasmus MC stands for a healthy population and excellence in healthcare. By conducting groundbreaking work, we aim to push boundaries through leading the way in research, education and healthcare. We have access to the latest equipment and techniques in a state-of-the-art environment.

The Systems Biology of Signaling and Transcription Factors group is part of the Department of Cell Biology, which houses innovative technologies and top-level facilities to support its research lines. The main focus of the department is on transcription regulation and genome organization in (neuro-) developmental and blood cell forming systems in order to understand cell fate determination and differentiation. The department is embedded in the dynamic, internationally competitive research community of over 40 biomedical research labs within the Erasmus MC. The main language of the research community is English. During the PhD training, our students follow specialized and multi-disciplinary lab courses organized by the Graduate School at Erasmus MC, often in collaboration with Medical Genetics Center (MGC), a collaboration with Leiden University and LUMC.