The laboratory of Molecular Stem Cell Biology1 at the Center for Lysosomal and Metabolic Diseases2, Departments of Clinical Genetics3 and Pediatrics4 of the Erasmus University Medical Center in Rotterdam, The Netherlands, embedded within the research school MGC (Medical Genetics Centre South West Netherlands and the Erasmus MC Graduate school5,6, is searching for a highly motivated candidate to join our group to develop lentiviral gene therapy for lysosomal storage diseases and to study the molecular mechanisms involved.

Lysosomal storage diseases are a group of ~50 genetic diseases in which one of the lysosomal enzymes responsible for degradation of macromolecules is defective. This results in accumulation of substrates in lysosomes, lysosomal pathology, and eventually cell death. Patients with a lysosomal storage disease have symptoms in multiple tissues and organs including the central nervous system, muscle, cartilage, bone, and visceral organs. We have generated iPSC-based cell models from patients with lysosomal storage diseases, and we are recapitulating pathology in vitro. These models are used to study the mechanisms by which enzyme deficiency leads to pathology, and for developing treatment options based on personalized medicine. In particular, we developed 3D-tissue engineered skeletal muscle on a chip and an innovative platform to measure contractile force in collaboration with the company Optics11.

We use a variety of techniques in the field of tissue engineering, molecular biology, biochemistry, cell biology and pathology, including cellular reprogramming to iPSCs, gene editing using CRISPR/Cas9, immunofluorescent and electron microscopy analysis, and genome-wide expression analysis including RNAseq and quantitative proteomics. Our research has strong links with the clinic, in which patients are treated and assessed. We collaborate with various national and international partners and are member of the European Reference Networks EURO-NMD and metabERN.

1. https://www.erasmusmc.nl/en/sophia/research/groups/molecular-stem-cell-biology-of-lysosomal-storage-diseases
2. https://www.erasmusmc.nl/en/sophia/research/group/lysosomal-and-metabolic-diseases-clinical-research
3. https://www.erasmusmc.nl/research/departments/clinical-genetics
4. https://www.erasmusmc.nl/en/sophia/patient-care
5. http://www.medgencentre.com/
6. https://www.eur.nl/en/erasmusmc/graduate-school

A background in molecular biology/cell biology is required, but specific experience with the techniques mentioned is not. We seek a candidate with a MSc or PhD degree with an interest in molecular medicine, and who is enthusiastic, ambitious, a good team player and has good level of speaking and writing English. Candidates applying as Postdoctoral researcher should have at least one manuscript published or in submission in an international peer-reviewed journal.

• We offer a position for 4 years (PhD student) or 3 years (post-doc) according to the gross
• Monthly salary amounts to a maximum of € 3.336,- (OIO-scale) or € 3.652,- (10-4), depending on your level of education and relevant experience and based on a full-time working week of 36 hours.
• Excellent fringe benefits, such as a 13th month that is already paid out in November and an individual travel expense package.
• Pension insurance with ABP. We take care of approximately 2/3 of the monthly contribution.
• Special benefits, such as a incompany physiotherapist and bicycle repairer. And there is also a gym where you can work on your fitness after work.

Erasmus MC

Erasmus MC stands for a healthy population and excellence in healthcare. By conducting groundbreaking work, we aim to push boundaries through leading the way in research, education and healthcare. We have access to the latest equipment and techniques in a state-of-the-art environment.

The project will be performed within the research group Molecular Stem Cell Biology at the Departments of Pediatrics and Clinical Genetics of the Erasmus MC, which are part of the Center for Lysosomal and Metabolic Diseases, a multidisciplinary center for patient treatment, clinical and basic/applied research.