Postdoc in CRISPR technologies

The group of Dr. Harsha Devalla investigates fundamental mechanisms regulating heart development and disease, with particular emphasis on how heart rhythm is established at the cellular and molecular levels. We use state-of-the-art model systems including human induced pluripotent stem cell (hiPSC)-derived cardiomyocyte subtypes and autonomic neurons, as well as 3D cardiac assembloids. We seek a molecular engineer for a project aimed at identifying genetic regulators of heart rate and rhythm.

This role is ideal for candidates who bring a strong wet-lab background in molecular cloning and genetic engineering together with solid computational skills for functional genomic screen design and analysis. You will build CRISPR tools, design optimized pooled genetic screens (e.g. Perturb-seq–based approaches), and troubleshoot the experimental workflows. Your expertise will combine cutting-edge tool development, including molecular cloning, lentiviral vector and library design, and the computational analysis of high-content transcriptomic and phenotypic data.

Additional responsibilities include:

• participation in grant and grant report writing;
• teaching and mentoring interns and lab members;
• engaging in overall lab operations including maintaining inventories and creating/updating standard operating procedures.

We invite applications for a postdoctoral researcher to lead the development and implementation of CRISPR-based technologies for functional genomics in cardiac and neuro-cardiac systems. You have:

• a PhD in molecular biology, molecular genetics, bioengineering or a closely related discipline with a focus on molecular engineering and synthetic biology;
• demonstrated expertise in molecular cloning, genetic engineering, and the design of advanced CRISPR tools, with clear evidence of developing or significantly optimizing CRISPR-based technologies such as multiplexed gRNA libraries, vector systems or delivery strategies;
• proven ability to design, execute, and troubleshoot pooled and/or arrayed CRISPR screens in mammalian cells;
• strong computational skills in bioinformatics and data analysis (e.g. R, Python or equivalent) for genomic and transcriptomic screening datasets.

Moreover, you are passionate about science and have a meticulous eye for detail. You are enthusiastic about interdisciplinary collaboration, enriching the diverse expertise in the group. With strong communication skills, a team-oriented mindset, and a proactive attitude, you thrive in a dynamic research environment.

• A flying start to your career in scientific research.
• Plenty of room for your drive to shape tomorrow's healthcare.
• Working on large-scale and in-house research, with motivated colleagues from all over the world.
• You will be employed by Amsterdam UMC Research BV.
• A contract for 12 months, with the intention to extend.
• Salary scale 10: € 3.598 to € 5.669 gross based on full-time employment (depending on education and experience) and a year-end bonus of 8.3%. Calculate your net salary here.
• Holiday hours: 190.4 per year for fulltime and a possibility to save additional hours.
• Pension accrual with BeFrank, a modern, comprehensible and fairly priced pension.
• For >7 km each way, 100% reimbursement for public transport travel costs and, for private transport, €0.18 per km up to a maximum of 40 km each way.
• Do you prefer walking or cycling? Take advantage of our good bike scheme. Moreover, you will receive a reimbursement of €0.18 per km.

Watch this video with more information about joining Amsterdam UMC Research BV.

Amsterdam UMC Research BV supports non-profit scientific research. In doing so, we provide researchers with everything they need to excel. Our principal investigators (PIs) and project leaders offer support in the field of project management, finance and human resources. In medical scientific research projects, legal support is also provided.

Watch the video to find out more.

Dr. Devalla's group is based in the department of Medical Biology at location AMC of the Amsterdam UMC. We strive for a supportive, collaborative environment promoting cross-pollination of ideas among researchers with knowledge of the cardiovascular system, stem cell biology, tissue engineering, molecular and phenotypic assays. There is strong complementarity with other PI-groups in the department working on in vivo models and gene therapies for cardiac repair.