PhD cell therapy and next-generation retinal organoids

This PhD project aims to develop next-generation human retinal models to better understand photoreceptor diseases and accelerate regenerative therapies for vision loss. It is embedded within and supported by the national Lifelong VISION (LLV) program, funded through an NWO Gravitation grant, which aims to develop innovative treatments for inherited retinal diseases and age-related macular degeneration. The project combines stem cell biology, 3D bioprinting, and tissue engineering to build advanced, in vitro models of the outer retina. Vascularized retinal pigment epithelium (RPE)–choroid constructs will be combined with photoreceptors derived from induced pluripotent stem cells (iPSCs). These models will allow you to dissect the functional and structural interactions between photoreceptors, RPE cells, and vascular cells under both healthy and disease-relevant conditions.

The project provides an opportunity to contribute to cutting-edge translational research with real potential to impact future therapies for blindness.

Would you like to know more about the different phases within the PhD trajectory? You can read more about this on this page.

As a PhD student, you will focus on:

• developing next-generation retinal organoids;
• generating 3D bioprinted retinal tissues to study RPE–photoreceptor–endothelial interactions under controlled conditions;
• characterizing organoids and bioprinted tissues using molecular, cellular, and functional assays (e.g., immunostaining, gene expression analysis, imaging, electrophysiology, or other functional readouts);
• generating patient-specific iPSC-derived photoreceptors to model disease phenotypes and investigate degeneration mechanisms;
• evaluating regenerative strategies, including photoreceptor cell therapy, gene therapy, and small-molecule interventions, to restore photoreceptor function and assess tissue integration;
• collaborating with interdisciplinary teams, including experts in iPSC technology, retinal biology, and translational regenerative medicine;
• publishing in international peer-reviewed journals and presenting findings at conferences.

For this project, we are seeking a motivated PhD candidate with a strong interest in regenerative medicine, stem cell and organoid technologies, and retinal research. Moreover, you:

• have a completed MSc (or equivalent) in Cell biology, Molecular biology, Biomedical sciences, Neuroscience, Ophthalmic research, or related field;
• preferably have experience with stem cell cultures (iPSCs), 3D culture or organoid systems, bioprinting, molecular and cellular biology assays, imaging, and/or functional characterization;
• have excellent English communication skills;
• work well independently as well as collaboratively in a multidisciplinary team;
• have the ambition to complete a PhD thesis and develop as a researcher.

Candidates with additional experience in translational research, preclinical studies, or cell therapy are strongly encouraged to apply.

• Access to state-of-the-art facilities for stem cell research, 3D bioprinting, organoid culture, and retinal biology.
• Mentorship from experienced Principal Investigators with expertise in retina, iPSC technology, and translational research.
• Opportunity to shape your own research direction within the theme of next-generation retinal organoids and RPE–photoreceptor–endothelial interactions in 3D tissues.
• A stimulating, collaborative, and inclusive work environment with multidisciplinary expertise.
• You will be employed by Amsterdam UMC Research BV.
• A contract for 12 months, with the intention to extend for a total of 4 years. The Guideline PhD contract applies to this vacancy.
• Salary scale OIO: € 3.108 to € 3.939 gross based on full-time employment (depending on education and experience) and a year-end bonus of 8.3%. Calculate your net salary here.
• Holiday hours: 190.4 per year for fulltime and a possibility to save additional hours.
• Pension accrual with BeFrank, a modern, comprehensible and fairly priced pension.
• For >7 km each way, 100% reimbursement for public transport travel costs and, for private transport, €0.18 per km up to a maximum of 40 km each way.
• Do you prefer walking or cycling? Take advantage of our good bike scheme. Moreover, you will receive a reimbursement of €0.18 per km.

Watch this video with more information about joining Amsterdam UMC Research BV.

Amsterdam UMC Research BV supports non‑profit scientific research by providing researchers with the infrastructure and expertise to excel, including project management, finance, HR, and legal support.

Watch the video to find out more.

The Amsterdam UMC Ophthalmogenetics section focuses on genetic eye diseases, combining clinical DNA diagnostics with advanced laboratory research and training of young medical and scientific talent. The group generates stem‑cell–derived retinal cells and organoids to study disease mechanisms, test therapies, and develop gene‑ and cell‑based treatments, using 3D bioprinting to create complex retinal tissue models. For more details, see the group’s research overview: https://ophthalmogenetics.nl/over-onderzoek

Lifelong VISION (LLV) is a national Gravitation research program funded by the Dutch Ministry of Education, Culture and Science that develops innovative therapies to combat vision loss from inherited retinal diseases and age‑related macular degeneration. The consortium focuses on gene therapy, pathway‑targeted treatments, and cell‑based approaches, and uses advanced lab and in vivo models to test these strategies. LLV also leverages artificial intelligence to match patients with the most promising treatments at the right time. Learn more about the program here: https://www.lifelongvision.nl/

LLV brings together ten leading research institutions and is led by Prof. Dr. Caroline Klaver (Radboudumc & Erasmus MC), with partners including Amsterdam UMC, University of Twente, Hubrecht Institute, Eindhoven University of Technology, University of Amsterdam, Utrecht University, Tilburg University, and the Netherlands Institute for Neuroscience.

How do I apply? Please submit your CV, motivation letter (highlighting your interest and relevant experience), and contact details of at least two references to dr. Mitra Farnoodian Tedrick by hitting the ‘Apply now’ button. In your motivation, describe your interest in retinal research, organoid development, 3D bioprinting, and cell therapy.

During the publication period, applications will be handled continuously. If the vacancy is filled, it will be closed prematurely.

If you have any questions about this position or the project, please feel free to contact dr. Mitra Farnoodian Tedrick at m.farnoodian@amsterdamumc.nl.

For more information about the application procedure, please contact Rhiannon Sandfort, recruitment advisor, at r.e.sandfort@amsterdamumc.nl.

A reference check, screening and hiring assessment may be part of the procedure. Read here whether that applies to you. If you join us, we ask you for a VOG (Certificate of Good Conduct).

Internal candidates will be given priority over external candidates in case of equal suitability.

Acquisition in response to this vacancy will not be considered.