Genome sequencing has become a standard technology in biomedical research. The technique is used to diagnose inherited diseases, target treatments, and identify health risks. Certain gene variants can increase a person's risk of diseases such as cancer and heart conditions. This technology makes use of innovative reagents, in combination with an optical microscope. A major drawback of this technology is that it only reveals the genetic baseline of biological processes.

The ability to measure the make-up of not only the genome but of proteins and protein expression, would create an enormous scientific opportunity as proteins are at the basis of all biological processes. We envision the realization of such protein sequencing methodology by building on recent developments in super-resolution microscopy. Super-resolution microscopy allows for circumventing the diffraction limit, to visualize otherwise invisible details within the cell. Super-resolution microscopy combines clever optical microscope designs followed by sophisticated image reconstruction.

During this Ph.D. project, the student will work on the development of optical techniques for protein sequencing. This can be achieved through an integrated approach that combines estimation theory, control of the illumination light of the microscope in space and time, and extremely fast and sensitive detection of the emitted light at a nanoscopic time scale. In this Ph.D. project, you will develop novel optical designs, statistical image analysis tools, and advanced control strategies. You will realize the sequencing of proteins and charter unknown scientific territory by deciphering biological processes one molecule at a time.

Departments

Precision and Microsystems Engineering

The Department of Precision and Microsystems Engineering (PME) carries out research and provides education in the field of high-tech systems and scientific instrumentation. We aim to solve fundamental questions in engineering science to advance the performance of precision systems and devices as well as their design. Our specific mission is to merge mechanical engineering concepts with micro and nanoscience. Increasing miniaturization and functionality along with improving sensitivity, speed, and reliability are the key topics in our work.

Delft Center for Systems and Control

The department Delft Center for Systems and Control (DCSC) of the faculty of Mechanical, Maritime, and Materials Engineering coordinates the education and research activities in systems and control at Delft University of Technology. The center's research mission is to conduct fundamental research in systems dynamics and control, involving dynamic modeling, advanced control theory, optimization, and signal analysis. The research is motivated by advanced technology development in physical imaging systems, renewable energy, robotics, and transportation systems.

• Experience in programming
• Passion for tinkering with experimental hardware and code
• Strong interpersonal and communications skills (verbal and written) in English
• A master’s degree or equivalent in physics with courses on optics.

Doing a PhD at TU Delft requires English proficiency at a certain level to ensure that the candidate is able to communicate and interact well, participate in English-taught Doctoral Education courses, and write scientific articles and a final thesis. For more details please check the Graduate Schools Admission Requirements.

Doctoral candidates will be offered a 4-year period of employment in principle, but in the form of 2 employment contracts. An initial 1,5 year contract with an official go/no go progress assessment within 15 months. Followed by an additional contract for the remaining 2,5 years assuming everything goes well and performance requirements are met.

Salary and benefits are in accordance with the Collective Labour Agreement for Dutch Universities, increasing from € 2443 per month in the first year to € 3122 in the fourth year. As a PhD candidate you will be enrolled in the TU Delft Graduate School. The TU Delft Graduate School provides an inspiring research environment with an excellent team of supervisors, academic staff and a mentor. The Doctoral Education Programme is aimed at developing your transferable, discipline-related and research skills.

The TU Delft offers a customisable compensation package, discounts on health insurance and sport memberships, and a monthly work costs contribution. Flexible work schedules can be arranged. For international applicants we offer the Coming to Delft Service and Partner Career Advice to assist you with your relocation.

Delft University of Technology

Delft University of Technology is built on strong foundations. As creators of the world-famous Dutch waterworks and pioneers in biotech, TU Delft is a top international university combining science, engineering and design. It delivers world class results in education, research and innovation to address challenges in the areas of energy, climate, mobility, health and digital society. For generations, our engineers have proven to be entrepreneurial problem-solvers, both in business and in a social context. At TU Delft we embrace diversity and aim to be as inclusive as possible (see our Code of Conduct). Together, we imagine, invent and create solutions using technology to have a positive impact on a global scale.

Challenge. Change. Impact!

Faculty Mechanical, Maritime and Materials Engineering

The Faculty of 3mE carries out pioneering research, leading to new fundamental insights and challenging applications in the field of mechanical engineering. From large-scale energy storage, medical instruments, control technology and robotics to smart materials, nanoscale structures and autonomous ships. The foundations and results of this research are reflected in outstanding, contemporary education, inspiring students and PhD candidates to become socially engaged and responsible engineers and scientists. The faculty of 3mE is a dynamic and innovative faculty with an international scope and high-tech lab facilities. Research and education focus on the design, manufacture, application and modification of products, materials, processes and mechanical devices, contributing to the development and growth of a sustainable society, as well as prosperity and welfare.

Click here to go to the website of the Faculty of Mechanical, Maritime and Materials Engineering. Do you want to experience working at our faculty? This video will introduce you to some of our researchers and their work.