Strikingly, a single molecule, named ppGpp, orchestrates decisions on growth and survival across bacterial species. Deciphering how this molecule encodes information in real-time and in living cells is currently impossible. In this project, we will set out to develop a biosensor based on fluorescence for direct measurements of this molecule in living bacteria. We will use state-of-the-art methods to generate and functionally screen large libraries of sensor candidates, using a combination of molecular cloning, flow cytometry and microscopy techniques.

For this project, we are looking for a junior laboratory technician that will be based at the VU in Amsterdam for a maximum duration of 1 year.

Your duties

• Perform molecular cloning based on PCR to generate variants of fluorescent sensors
• Transform bacteria (E. coli) with sensor variants
• Screen functionality of sensor variants using a combination of flow cytometry and microscopy
• Maintain a detailed digital lab journal to record activities and results.

• Recent HLO graduate (or expected to graduate shortly after commencement of project) or equivalent experience.
• You have good experimental skills, with experience in molecular cloning and microscopy a plus.
• You can communicate in English, both verbally and in writing.

Fixed-term contract: 1 year.

A challenging position in a socially involved organization and dynamic research group.
On full-time basis the remuneration amounts to a minimum gross monthly salary of €2,818 (scale 8) and a maximum €3,764 (scale 8), depending on your education and experience. The job profile (Education/ Research Technician 4) is based on the university job ranking system and is vacant for at least 0.8 FTE.

The employment contract will be for a maximum of 1 year.

Additionally, Vrije Universiteit Amsterdam offers excellent fringe benefits and various schemes and regulations to promote a good work/life balance, such as:

• A maximum of 41 days of annual leave based on full-time employment
• 8% Holiday allowance and 8.3% end-of-year bonus
• Solid pension scheme (ABP)
• Contribution to commuting expenses.

Vrije Universiteit Amsterdam

The ambition of Vrije Universiteit Amsterdam is clear: to contribute to a better world through outstanding education and ground-breaking research. We strive to be a university where personal development and commitment to society play a leading role. A university where people from different disciplines and backgrounds collaborate to achieve innovations and to generate new knowledge. Our teaching and research encompass the entire spectrum of academic endeavor - from the humanities, the social sciences and the natural sciences through to the life sciences and the medical sciences.

Vrije Universiteit Amsterdam is home to more than 30,000 students. We employ over 5,500 individuals. The VU campus is easily accessible and located in the heart of Amsterdam's Zuidas district, a truly inspiring environment for teaching and research.

Diversity
We are an inclusive university community. Diversity is one of our most important values. We believe that engaging in international activities and welcoming students and staff from a wide variety of backgrounds enhances the quality of our education and research. We are always looking for people who can enrich our world with their own unique perspectives and experiences.

The Faculty of Science
The Faculty of Science inspires researchers and students to find sustainable solutions for complex societal issues. From forest fires to big data, from obesity to medicines and from molecules to the moon: our teaching and research programmes cover the full spectrum of the natural sciences. We share knowledge and experience with leading research institutes and industries, both here in the Netherlands and abroad.

Working at the Faculty of Science means working with students, PhD candidates and researchers, all with a clear focus on their field and a broad view of the world. We employ more than 1,250 staff members, and we are home to more than 11,000 students.

About the A-LIFE Institute
The mission of A-LIFE is to advance the fundamental understanding of the interplay between life and its environment through innovative and curiosity-driven science. The department seeks to connect properties and emergent behaviours from micro to global scales, from molecules to ecosystems, including human populations. We aim to inspire our students and apply our expertise to shape a sustainable future and support healthy living.