We are looking for an enthusiastic research technician with talent for tissue engineering and biochemistry. Do you have interest in generating heart and muscle models from patient's skin cells and use them to investigate the molecular mechanisms of rare diseases? Then join us in our state-of-the-heart laboratory, where we combine cutting-edge analytical technologies and advanced tissue models to understand rare metabolic cardio-/myopathies and find potential treatments.

This position offers the opportunity to enrich your technical skill-set while working in an international, dynamic and goal-driven team, focused on the study of rare metabolic disorders. The team is hosted at the Translational Metabolic Laboratory (TML, RadboudUMC), an interdisciplinary department that combines research and diagnostics, which provides the ideal setting for translational projects with potential to significantly impact patients' healthcare.

This position is part of a national, multi-center project funded by the Prinses Beatrix Spierfonds, focused on bridging -OMICS technologies and 2D/3D-muscle and -heart models to develop a novel platform for preclinical treatment discovery targeting rare cardio-/myopathies. This project represents a collaborative effort led by the RadboudUMC (TML, Nijmegen), and involving the ErasmusMC (Dept. of Pediatric Metabolic Diseases, Rotterdam) and the University of Twente (Dept. of Applied Stem Cell Technologies, Enschede).

As our research technician, you will:

• Provide support for the culture and maintenance of human induced pluripotent stem cells (hiPSCs) derived from healthy donors and patients affected by rare congenital cardio-/myopathies.
• Implement protocols to differentiate control and patient-derived hiPSCs into muscle and heart cells.
• Develop qPCR- and immunofluorescence-based protocols for characterization of heart and muscle models.
• ontribute to the molecular profiling of control and patient-derived muscle and heart models via mass spectromtery-based -OMICS (metabolomics, proteomics).

We are looking for a research technician who excels in accuracy and flexibility, employing a project-based approach driven by scientific curiosity. Your are a true team player, proficient in pursuing common goals and motivating others.

Moreover you have:

• A hbo or hlo HLO degree, or a Master degree in disciplines relevant for the position (e.g. biochemistry, tissue engineering, biotechnology, biomedical sciences, medical/human biology).
• Familiarity with biochemistry and related analytical technologies (especially mass-spectrometry).
• Familiarity with (stem) cell culture, handling and differentiation.
• Demonstrated fluency in English, as you will be part of an international group and will be involved in (inter)national collaborations.

Fixed-term contract: 1 year with a possible extension.

At Radboud university medical center, you build on your future. We are committed to providing the best care, education, and research. And we are true to our word, because we help you develop and seize opportunities and give you the room to grow. As an employer, we believe that employees should feel vital and happy at work in all stages of life. We are also committed to creating a healthy and safe working environment. Our employment conditions contribute to that. What we offer:

• A gross monthly salary in scale 7 between € 2.819 and € 3.772 (for a full-time appointment). You will also receive an 8% holiday allowance, an 8.3% end-of-year bonus, and a 47% to 72% bonus for working unsocial hours.
• Plenty of opportunities for personal development. You can take a variety of courses in our online learning environment. You can also share knowledge with colleagues through digital learning spaces – perfect for getting inspired and inspiring others.
• Your well-being and vitality are our priorities. For instance, you can enjoy a 40% discount on a sports subscription at the Radboud Sports Center along with access to the Healthy Professionals program designed to assist you in managing your energy. If you are going through a life-changing event, our Company Support Team and a personal coach are there to help you.
• If you are going through a life-changing event, our Company Support Team and a personal coach are there to help you.
• Support in achieving a good work-life balance at every stage of your life. This includes advice and training courses (from ‘Millennial Dilemmas’ to ‘Your Career After 57’), activities at our own mindfulness center, and informal caregiving consultations if you have questions about juggling work and caregiving responsibilities at home.
• 168 holiday hours per year (23 days) based on a 36-hour working week.
• 26 weeks of parental leave, nine of which are paid.
• You build up a pension by ABP Pension Fund, and Radboudumc pays 70% of the pension premium.
• Discounts on health insurance and ten other types of insurance, from home insurance to legal assistance.
• An allowance for your travel expenses or a working-from-home allowance of €2 per day.

Radboudumc

The Translational Metabolic Laboratory (TML) is a hospital-based laboratory, part of the Department of Human Genetics of the Radboud University Medical Center. TML is dedicated to the discovery, development and validation of novel personalized diagnostics and innovative treatments for inborn errors of metabolism, neurodegenerative/neurometabolic diseases, iron disorders, pediatric nephrology and oncology.

The laboratory, with its 11 staff-members and over 100 laboratory personnel, has a strong history in translational molecular medicine research with considerable scientific output (160 peer-reviewed papers annually). The laboratory hosts state-of-the-art analytical equipment for proteomics, metabolomics and glycomics, and multiple targeted diagnostic platforms to translate research findings to patient-focused diagnostics. TML is also hosting the Radboudumc Technology Center (RTC) of Mass Spectrometry, and is a frequent partner in service and in scientific (inter)national consortia.

Radboudumc
Radboud university medical center is a university medical center for patient care, scientific research, and education in Nijmegen. Radboud university medical center strives to be at the forefront of shaping the healthcare of the future. We do this in a person-centered and innovative way, and in close collaboration with our network. We want to have a significant impact on health and healthcare. We want to improve with each passing day, continuously working towards better healthcare, research, and education. And gaining a better understanding of how diseases arise and how we can prevent, treat, and cure them, day in and day out. This way, every patient always receives the best healthcare, now and in the future. Because that is why we do what we do.