Our group aims to attain broader relevance of CAR-T cell therapy through improvement of tumor targeting and tumor elimination, overcoming resistance, minimization of off-tumor toxicity and expansion of the availability across histocompatibility barriers. We use iPSCs as an unlimited source of off-the-shelf CAR-T cells and have developed a unique expertise in iPSC differentiation towards the lymphoid lineage.
In this project we aim to develop the technological tools for efficient and clinically compatible production of next generation, multi-engineered, effective, off-the-shelf iPSC-derived CAR-T cells. We envision that the success of our research will lay foundation for translation to clinical studies towards more effective, safer and broader application of CAR-T cells.
You will work in a dedicated team of postdocs, PhD students and technicians and you will be supervised by an expert in the field of CAR-T cell therapy, furthermore:
- You will be mainly working in a BSL-II tissue culture laboratory and your main activities will be: culturing of iPSC and other cell lines, production of viral vectors, appling protocols for directed differentiation of iPSC, culture of primary T cells and transduction for the generation of CAR-T cells;
- You will employ gene-editing methodologies (CRISPR/Cas9, TALEN);
- You will be performing molecular cloning and generating new plasmid vector constructs;
- You will be performing multi-color flow cytometry.