Research Technician neuronal cell culture and antibody generation

Research Technician neuronal cell culture and antibody generation

Published Deadline Location
yesterday 25 Jul Utrecht
We are looking for a technician to join our team focusing on the role of complement in neuropathies, and the UMAB facility for generation of new monoclonal antibodies.

Job description

We offer a position for a research technician to join our complement team to set up co-cultures of neuronal cells, and the UMAB facility for the generation of new monoclonal antibodies.

A technician (HBO) trained in cellular (cell culture, transfection/transduction, flow cytometry) and molecular biological techniques.

You do not need to have years of experience, as long as you can work accurate and are flexible and like to learn new techniques. Affinity with (long) cell cultures is a must.

Specifications

University Medical Center Utrecht (UMC Utrecht)

Requirements

You are a teamplayer and are eager to learn. You have a high efficiency and reproducibility level in the lab. A professional level of proficiency in English (written and spoken) is recommended. You can think analytically and have problem-solving skills. You will work in an enthusiastic group of 20 people, post-docs, technicians and PhD students.

Conditions of employment

The maximum salary for this position (36 - 36 hours) is € 4.214,00 gross per month based on full-time employment.

In addition, we offer an annual benefit of 8.3%, holiday allowance, travel expenses and career opportunities. The terms of employment are in accordance with the Cao University Medical Centers (UMC).

Department

The technician will be part of the Antibody Therapy group led by Professor Jeanette Leusen at UMC Utrecht. You will collaborate closely with our team researching the role of complement in neuroinflammatory diseases. Specifically, you will work alongside the senior technician of the iPCS lab, to establish neuronal co-cultures with iPSC-derived motor neurons, sensory neurons, Schwann cells, and astrocytes. Our methods include iPSC cultures, immunohistochemistry and microscopy to evaluate these cell types. All individual iPSC cultures are operational, and all stainings required to characterize these cell types are in place. The complement team will utilize these co-cultures to further investigate complement activation within various neuroinflammatory disease contexts.
Furthermore, you will work closely with the senior technician of the UMCU Monoclonal antibody facility UMAB. UMAB is the facility of the CTI, Center for Translational Immunology of the UMCU, but also the facility for ONCODE researchers. UMAB can generate new monoclonal antibodies on request, ranging for antibodies used for research, diagnostics or therapy. We use a unique cellular immunization strategy of mice, to produce high affinity, very specific antibodies, Screening of serum and hybridoma titers will be done by FACS (flow cytometry), and hybridomas will be cultured, subcloned end sequenced, so antibodies can be produced recombinantly. All these techniques are up and running within the UMAB facility.

Additional information

Contact our colleague:
Danielle Krijgsman
06 11 23 86 60
d.krijgsman-4@umcutrecht.nl

Specifications

  • Technical and laboratory
  • Health
  • max. 36 hours per week
  • max. €4214 per month
  • Higher professional education
  • 2024-4932

Employer

University Medical Center Utrecht (UMC Utrecht)

Learn more about this employer

Location

Heidelberglaan 100, 3584CX, Utrecht

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Application procedure

Application procedure

Make sure to apply no later than 25 Jul 2024 23:59 (Europe/Amsterdam).