You will be involved in multiple ongoing research projects in the recently started research group of Dr. Noordermeer. In addition to experimental work, you will be involved in lab management tasks related to setting up and running a new research lab. The group of Dr. Noordermeer studies the role of BRCA1 and its protein complexes in maintaining genomic stability and how mutations in this gene affect complex formation and hence cancer onset and progression. The lab uses a variety of proteomic, genomic and dedicated functional assays and you will be involved in the generation of cell line models via CRISPR-Cas9 and the development of assays to study protein recruitment to damaged DNA.

In short:

• You received your BSc (HLO laboratory research) in molecular biology or a related field;

• You have hands-on experience with standard molecular biology techniques;

• You have an organized and independent work style;

• You have great sense of teamwork and excellent communication skills;

• You are motivated to study BRCA1 function in the maintenance of genomic stability.

You are an enthusiastic and driven research technician who likes to work in a young and dynamic team with a focus on unravelling fundamental and mechanistic research questions. You have hands-on experience in common molecular biology tools (molecular cloning, mammalian cell culture, Western blotting, fluorescent microscopy, flow cytometry, etc). Experience with genome editing (CRISPR/Cas9) and DNA damage repair is an advantage. You are accurate and able to work independently and within a team. You have a flexible attitude, great sense of teamwork and possess excellent communication skills in English, and preferably also in Dutch. Furthermore, you like to be involved in the organisational tasks of running a research lab.

Fixed-term contract: 1 year.

You will be employed on the basis of 36 hours per week. Appointment is for one year, with with an extension of two years. Your salary is based on your education and experience and is a maximum of  € 3,099 gross per month for a full time position (scale 7 in the Collective Labour Agreement forUniversity Hospitals).

LUMC

At the Leiden University Medical Center, we continuously work on improving patient care. We invest in groundbreaking, international research and work with the latest equipment. Together with our team of doctors, medical specialists, teachers, academic researchers and supporting staff, we aim for the best quality in health care, education and international research. And we need you to realize our goals!

http://www.lumc.nl/

Department of Human Genetics

The Department of Human Genetics is a broadly oriented research department, led by Professor Silvère van der Maarel, focusing on the genetic aspects of a variety of diseases, such as cancer, and the translation into clinical relevance. The department consists of 22 research units, embedded in four overarching research themes. You will work in the recently established research group led by Dr. Sylvie Noordermeer, which is embedded in the research theme ‘Genome instability and cancer’ and has a clear focus on better understanding the fundamental mechanisms of DNA damage repair to improve clinical management of cancer patients. More specifically, the research in the Noordermeer lab governs the study of the protein BRCA1 and its regulation and function in multiple protein complexes involved in homologous recombination and genome stability maintenance. We complement our fundamental research in mammalian cell line models with studies on clinical data of BRCA1-mutated tumours to enable better risk prediction of the wide variety of BRCA1 mutations present in tumours. We have previously uncovered mechanisms involved in therapy resistance of BRCA1-deficient cells to PARPi (Noordermeer et al., Nature, 2018).